Dynamic Properties of Degenerated Intervertebral Disc Can Be Better Recovered by Elevated Protein Crosslinking
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چکیده
INTRODUCTION: The feasibility of using crosslinking reagent, e.g., the genipin or methylglyoxal, to enhance the biomechanical functions of the injured or degenerated intervertebral disc has been extensively investigated. Our previous study found that genipin could recover the disc dynamic properties of mild denatured disc; however, the recovery efficiency was degraded by fatigue loading. Genipin is able to crosslink the intrahelical, interhelical and intermicrofibrillar free amino peptides in collagen-based biomaterials. The degraded recovery efficiency post fatigue loading may suggest the insufficient crosslinking within the disc, especially for the intermicrofibrillar structures. The degree of crosslinking can theoretically be elevated by increasing the genipin concentration and crosslinking reaction time. The purpose of this study is to evaluate the effect of enhanced crosslinking on the recovery of dynamic properties of denatured disc post fatigue loading.
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